fopflash (Addgene inc)
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Addgene inc
fopflash
Fopflash, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 258 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/m51+super+8x+fopflash/M51+Super+8x+FOPFlash+(TOPFlash+mutant)+(Plasmid+%2312457)/pm41906239-113-16-17
Average 94 stars, based on 258 article reviews
Fopflash, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 258 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/m51+super+8x+fopflash/M51+Super+8x+FOPFlash+(TOPFlash+mutant)+(Plasmid+%2312457)/pm41906239-113-16-17
Average 94 stars, based on 258 article reviews
fopflash - by Bioz Stars,
2026-09
94/100 stars
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Plasmid Preparation:Article Title: CD73 restrains mutant β-catenin oncogenic activity in endometrial carcinomas Article Snippet: Wildtype and patient-specific exon 3 β-catenin plasmids were expressed using non-viral approaches (Lipofectamine 3000; Invitrogen) in all experiments. .. M50 Super 8x TOPFlash (Addgene plasmid #12456), Article Title: The developmental factor TBX3 engages with the Wnt/β-catenin transcriptional complex in colorectal cancer to regulate metastasis genes Article Snippet: .. M50 Super 8x TOPFlash (SuperTOPFlash is found at Addgene, Plasmid #12456) and Article Title: Programmable protein degraders enable selective knockdown of pathogenic β-catenin subpopulations in vitro and in vivo Article Snippet: .. On the day of transfection, each well received the following plasmids: M50 Super 8x TOPFlash plasmid (Addgene plasmid # 12456) or Article Title: Compound screening in human airway basal cells identifies Wnt pathway activators as potential pro-regenerative therapies Article Snippet: .. The following day cells were co-transfected with the pRL SV40 construct (E2231, Promega) using jetOPTIMUS (101000051, Polyplus) along with either the M50 Super 8x TOPFlash (deposited by Randall Moon, Addgene plasmid #12456 ; ) or the Article Title: Compound screening in primary human airway basal cells identifies Wnt pathway activators as potential pro-regenerative therapies Article Snippet: .. The following day cells were co-transfected with the M50 Super 8x TOPFlash (a gift from Randall Moon, Addgene plasmid #12456; http://n2t.net/addgene:12456 ; RRID:Addgene_12456), the Mutagenesis:Article Title: CD73 restrains mutant β-catenin oncogenic activity in endometrial carcinomas Article Snippet: Wildtype and patient-specific exon 3 β-catenin plasmids were expressed using non-viral approaches (Lipofectamine 3000; Invitrogen) in all experiments. .. M50 Super 8x TOPFlash (Addgene plasmid #12456), Article Title: Article Snippet: A total of 2 μg of DKK1 promoter and 0.5 μg of pRL (Promega, E2261) plasmids were transfected into SH-SY5Y cells (7.5×105) using FuGENE HD Transfection Reagent (Promega) in a 6-well culture plate. .. For the TCF/LEF luciferase reporter assay (TOPFlash), M50 Super 8x TOPFlash (Addgene, 12456) or Article Title: Programmable protein degraders enable selective knockdown of pathogenic β-catenin subpopulations in vitro and in vivo Article Snippet: .. On the day of transfection, each well received the following plasmids: M50 Super 8x TOPFlash plasmid (Addgene plasmid # 12456) or Article Title: Compound screening in human airway basal cells identifies Wnt pathway activators as potential pro-regenerative therapies Article Snippet: .. The following day cells were co-transfected with the pRL SV40 construct (E2231, Promega) using jetOPTIMUS (101000051, Polyplus) along with either the M50 Super 8x TOPFlash (deposited by Randall Moon, Addgene plasmid #12456 ; ) or the Article Title: Compound screening in primary human airway basal cells identifies Wnt pathway activators as potential pro-regenerative therapies Article Snippet: .. The following day cells were co-transfected with the M50 Super 8x TOPFlash (a gift from Randall Moon, Addgene plasmid #12456; http://n2t.net/addgene:12456 ; RRID:Addgene_12456), the Luciferase:Article Title: Article Snippet: A total of 2 μg of DKK1 promoter and 0.5 μg of pRL (Promega, E2261) plasmids were transfected into SH-SY5Y cells (7.5×105) using FuGENE HD Transfection Reagent (Promega) in a 6-well culture plate. .. For the TCF/LEF luciferase reporter assay (TOPFlash), M50 Super 8x TOPFlash (Addgene, 12456) or Reporter Assay:Article Title: Article Snippet: A total of 2 μg of DKK1 promoter and 0.5 μg of pRL (Promega, E2261) plasmids were transfected into SH-SY5Y cells (7.5×105) using FuGENE HD Transfection Reagent (Promega) in a 6-well culture plate. .. For the TCF/LEF luciferase reporter assay (TOPFlash), M50 Super 8x TOPFlash (Addgene, 12456) or Transfection:Article Title: Programmable protein degraders enable selective knockdown of pathogenic β-catenin subpopulations in vitro and in vivo Article Snippet: .. On the day of transfection, each well received the following plasmids: M50 Super 8x TOPFlash plasmid (Addgene plasmid # 12456) or Control:Article Title: Programmable protein degraders enable selective knockdown of pathogenic β-catenin subpopulations in vitro and in vivo Article Snippet: .. On the day of transfection, each well received the following plasmids: M50 Super 8x TOPFlash plasmid (Addgene plasmid # 12456) or Construct:Article Title: Programmable protein degraders enable selective knockdown of pathogenic β-catenin subpopulations in vitro and in vivo Article Snippet: .. On the day of transfection, each well received the following plasmids: M50 Super 8x TOPFlash plasmid (Addgene plasmid # 12456) or Article Title: Compound screening in human airway basal cells identifies Wnt pathway activators as potential pro-regenerative therapies Article Snippet: .. The following day cells were co-transfected with the pRL SV40 construct (E2231, Promega) using jetOPTIMUS (101000051, Polyplus) along with either the M50 Super 8x TOPFlash (deposited by Randall Moon, Addgene plasmid #12456 ; ) or the Article Title: Compound screening in primary human airway basal cells identifies Wnt pathway activators as potential pro-regenerative therapies Article Snippet: .. The following day cells were co-transfected with the M50 Super 8x TOPFlash (a gift from Randall Moon, Addgene plasmid #12456; http://n2t.net/addgene:12456 ; RRID:Addgene_12456), the Binding Assay:Article Title: B4GALT1 and Wntless collaborate to block LRP5/6 translocation from Golgi to cell surface. Article Snippet: After translation, low-density lipoprotein receptor–related protein 5/6, LRP5/6, are transported from ER through Golgi to cell surface, where they serve as the co-receptors of Wnt proteins to elicit the WNT/β-catenin signaling.. Here, Golgi-resident β-1, 4galactosyltransferase B4GALT1 is revealed to interact with LRP5/6, causing the Golgi retention of LRP5/6 and ultimately reducing LRP5/6 on the cell surface.. In addition to LRP5/6, B4GALT1 can also bind to the exclusive Wnt transporter Wntless. Negative Control:Article Title: B4GALT1 and Wntless collaborate to block LRP5/6 translocation from Golgi to cell surface. Article Snippet: After translation, low-density lipoprotein receptor–related protein 5/6, LRP5/6, are transported from ER through Golgi to cell surface, where they serve as the co-receptors of Wnt proteins to elicit the WNT/β-catenin signaling.. Here, Golgi-resident β-1, 4galactosyltransferase B4GALT1 is revealed to interact with LRP5/6, causing the Golgi retention of LRP5/6 and ultimately reducing LRP5/6 on the cell surface.. In addition to LRP5/6, B4GALT1 can also bind to the exclusive Wnt transporter Wntless. |
